SequenceAssembler

Advanced Protein Sequence Assembly from Mass Spectrometry Data

Unlock Protein Sequences with Precision

SequenceAssembler is a powerful, yet user-friendly software meticulously designed to reconstruct complete protein sequences. By intelligently integrating peptide-spectrum matching (PSM) and de novo sequencing data from mass spectrometry, it transforms complex raw data into clear, actionable biological insights.

Our intuitive interface, showcased below, simplifies the visualization and analysis of your proteomics data. Explore how SequenceAssembler differentiates peptide identifications (PSM in green, de novo in yellow) and illustrates the contribution of various enzymatic digestions to overall sequence coverage. The multi-template alignment view allows for robust comparison against reference sequences, clearly highlighting matches, mismatches, and experimentally covered regions—essential for accurate protein characterization and detailed domain analysis, such as identifying Albumin domains or antibody CDRs.

SequenceAssembler Screenshot 1 - Data Analysis Overview

Comprehensive data analysis view showing PSM and de novo contributions.

SequenceAssembler Screenshot 2 - Multi-template Alignment Visualization

Intuitive multi-template alignment for detailed sequence comparison.

Get SequenceAssembler & Example Data

Download the latest version of our software and explore example datasets to get started quickly.

Software requires .NET Framework (ClickOnce application). Example data includes sample files to test functionalities.